Fig. 5

CircRELN regulated RORA expression by targeting miR-1290. A The binding site between RORA 3’UTR and miR-1290 was analyzed by Targetscan. B and C The binding relationship between RORA and miR-1290 was confirmed by dual-luciferase reporter assay. D and E The expression of RORA in T98G and LN18 cells with miR-1290 enrichment was measured by qPCR and western blot. F and G The expression of RORA in tissue samples was measured by qPCR and western blot. H and I The expression of RORA in cell lines was measured by qPCR and western blot. J and K The expression of RORA in Sev-treated T98G and LN18 cells was detected by qPCR and western blot. L and M The expression of RORA in T98G and LN18 cells transfected with si-circRELN, si-NC, si-circRELN + anti-miR-NC or si-circRELN + anti-miR-NC was detected by qPCR and western blot. *P < 0.05