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Fig. 4 | BMC Anesthesiology

Fig. 4

From: Protective effects of dexmedetomidine on cerebral ischemia/reperfusion injury via the microRNA-214/ROCK1/NF-κB axis

Fig. 4

ROCK1 is the downstream target gene of miR-214. A Target genes of miR-214 predicted by Starbase, TargetScan, miRWalk and miRDB databases. B The mRNA expression of predicted target genes in brain tissues of rats injected with miR-214 inhibitor or NC inhibitor detected by RT-qPCR. C ROCK1 protein expression in brain tissues of rats injected with miR-214 inhibitor or NC inhibitor determined by immunohistochemistry. D Sequences for binding sites between miR-214 and ROCK1. E Luciferase activity of ROCK1-WT and ROCK1-MT after treatment of miR-214 mimic examined by dual-luciferase reporter assay. For panel C, comparison was made using unpaired t test. * p < 0.05 compared with rats injected with NC inhibitor. For panel B and E, comparisons were made using one-way or two-way ANOVA, respectively. * p < 0.05 compared with rats injected with NC inhibitor; # p < 0.05 compared with rats injected with NC mimic

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BMC Anesthesiology

ISSN: 1471-2253

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