Fig. 6

Dexmedetomidine modulated OGD-induced Cx43 reduction through PI3K-Akt-GSK-3β pathway. a, c, e, g Western blot of PI3K-Akt-GSK-3β signaling pathway. Representative images were shown. b, f Quantitative analysis of Akt and p-Akt expression. d, h Quantitative analysis of GSK-3β and p-GSK-3β expression. i Primary astrocytes were incubated with LY294002 or SB216763 for 1 h before OGD, then Cx43 was examined using Western blot. j Quantitative analysis of Cx43 expression with LY294002 or SB216763 pretreatment. Results were presented as mean ± SD (n = 6) and analyzed using One-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.001. LY294002, PI3K inhibitor. SB216763, GSK-3β inhibitor