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Fig. 5 | BMC Anesthesiology

Fig. 5

From: Sevoflurane modulates breast cancer cell survival via modulation of intracellular calcium homeostasis

Fig. 5

Effects of general anesthetics (GAs) on breast cancer cell proliferation. a Representative images showing the double immunostaining of cell nuclei with 4′,6-diamino-2-phenylindole (DAPI, blue, arrows) and 5-bromodeoxyuridine (BrdU, green, arrows) in control breast cancer cells (MCF 10A), estrogen receptor-positive (MCF 7) and negative (MDA-MB-436) breast cancer cells with and without sevoflurane (2%) and propofol (2 μM), at equipotent dose for 6 h. Scale bar = 100 μm. b Quantitative analysis of the effect of a 6-h exposure to an equipotent dose of sevoflurane (2%) vs. propofol (2 μM) on cell proliferation. Exposure to 2% sevoflurane and 2 μM propofol does not affect the percentage of cell proliferation (% of BrdU-positive cells) among all three cell lines (n = 12). All data are expressed as means ± SD from at least three separate experiments in duplicate or triplicate and analyzed by one-way ANOVA followed by Tukey multiple comparison tests

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