Fig. 5

miR-34a targeted the 3′-UTR of HDAC2. a Bioinformatics analysis showed that miR-34a has a binding site at the 3′-UTR of the HDAC2 mRNA. b DLRA was performed following co-transfection of a luciferase reporter that contained either a mutant (MU) or wild-type (WT) 3′-UTR from HDAC2 and the miR-34a mimic into HEK293T cells. The influence of the miR-34a on the luciferase activities of the MU and WT HDAC2 reporter constructs were examined. c, d WB and Q-PCR analyses showed the expression of HDAC2 expression in normal, CIVP, DEX, and vehicle groups at both mRNA and protein levels. e, f WB and Q-PCR analyses showed the expression of HDAC2 expression in CIVP and DEX with or without miR-34a inhibition at both mRNA and protein levels. Results are expressed as mean ± SD. *P < 0.05 as compared to the indicated groups. Abbreviations: CIVP, chronic inflammatory visceral pain; DLRA, dual luciferase reporter assay; DEX, dexmedetomidine; miRNA, micro ribonucleic acid; Q-PCR, quantitative polymerase chain reaction; TNBS, 2,4,6-Trinitrobenzenesulfonic acid; and WB, Western blot