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Fig. 3 | BMC Anesthesiology

Fig. 3

From: MiR-34a affects dexmedetomidine-inhibited chronic inflammatory visceral pain by targeting to HDAC2

Fig. 3

The effect of the inhibited expression of miR-34a on pain behaviors and pro-inflammatory cytokine production of CIVP rats. At 2 weeks after induction with TNBS, spinal cords were isolated from the normal rats and those transfected with adenovirus-miR-34a inhibitor or adenovirus-NC inhibitor. a Q-PCR was used to determine the levels of miR-34a in spinal cord tissues. b Rats were injected with Ad-NC inhibitor -GFP or Ad-miR-34a inhibitor-GFP with 1 × 109 PFU/mL. The expression of GFP was located and found on the transverse sections of spinal cord. Scale bar: 50 μm. c, d, e MWT, AWR, and TWL scores following DEX treatment and adenovirus infection. f Q-PCR was employed to detect mRNA levels of IL-6, IL-1β, and TNF-α and after DEX administration and adenovirus infection. Mean ± standard deviation (N = 3) was used to describe the data. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the normal group or the indicated group. Abbreviations: AWL, abdominal withdrawal reflex; CIVP, chronic inflammatory visceral pain; DEX, dexmedetomidine; miRNA, micro ribonucleic acid; MWT, mechanical withdrawal threshold; Q-PCR, quantitative polymerase chain reaction; TNBS, 2,4,6-Trinitrobenzenesulfonic acid; and TWL, thermal withdrawal latency

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