Fig. 6

Propofol attenuated TNF-α-modulated occludin expression via inhibiting Hif-1α/ VEGF/ VEGFR-2/ ERK signaling pathway in hCMEC/D3 cells. a Left side shows the image of a representative Western blot for Hif-1α, VEGF, p-VEGF in hCMEC/D3 cells that treated with TNF-α, ERK inhibitor (LY3214996, 5 μM) and propofol. Right side is the plot of normalized ratios of optical densities. b Left side shows the image of a representative Western blot for Hif-1α in hCMEC/D3 cells that treated with TNF-α, VEGFR-2 inhibitor (SU5416, 10 μM) and propofol. Right side is the plot of normalized ratios of optical densities. β-actin was served as internal loading control. Data were represented as the mean ± SD. *p < 0.05, compared with the control group. #p < 0.05, compared with TNF-α treatment group. Each assay was performed in five replicates