Fig. 5
The expression of Hif-1α was involved in the effects of propofol and TNF-α on occludin expression in hCMEC/D3 cells. a Left side shows the image of a representative Western blot for Hif-1α in hCMEC/D3 cells that treated with TNF-α and propofol. Right side is the plot of normalized ratios of optical densities. b The hCMEC/D3 cells were treated with TNF-α, propofol, and Hif-1α inhibitor (KC7F2, 10 μM). Left side shows the image of a representative Western blot for Hif-1α, VEGF, p-VEGFR-2, p-ERK, ERK and occludin. Right side is the plot of normalized ratios of optical densities. β-actin was served as internal loading control. Data were represented as the mean ± SD. *p < 0.05, compared with the control group. #p < 0.05, compared with TNF-α treatment group. Each assay was performed in five replicates