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Fig. 4 | BMC Anesthesiology

Fig. 4

From: Propofol attenuated TNF-α-modulated occludin expression by inhibiting Hif-1α/ VEGF/ VEGFR-2/ ERK signaling pathway in hCMEC/D3 cells

Fig. 4

The VEGF/VEGFR-2 signaling pathway was involved in the effects of propofol and TNF-α on occludin expression in hCMEC/D3 cells. a Left side shows the image of a representative Western blot for VEGF and p-VEGFR-2 in hCMEC/D3 cells that treated with TNF-α and propofol. Right side is the plot of normalized ratios of optical densities. b The hCMEC/D3 cells were incubated with TNF-α, propofol, and VEGFR-2 inhibitor (SU5416, 10 μM). Left side shows the image of a representative Western blot for VEGF, p-VEGFR-2, p-ERK, ERK and occludin in hCMEC/D3 cells. Right side is the plot of normalized ratios of optical densities. β-actin was served as internal loading control. Data were represented as the mean ± SD. *p < 0.05, compared with the control group. #p < 0.05, compared with TNF-α treatment group. Each assay was performed in five replicates

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