Fig. 1

TNF-α could decrease the expression of occludin in hCMEC/D3 cells. a The hCMEC/D3 cells were treated with different concentrations of TNF-α (1, 10, 25, 50 and 100 ng/ml) for 8 h. Left side shows the image of a representative Western blot for occludin; right side is the plot of normalized ratios of optical densities. b The hCMEC/D3 cells were incubated with TNF-α (10 ng/ml) for different hours (2, 4, 8, 12, 24 h). Left side is the image of a representative Western blot for occludin; right side is the plot of normalized ratios of optical densities. c The cell viability of hCMEC/D3 cells after TNF-α treatment. β-actin was served as internal loading control. Data were represented as the mean ± SD. *p < 0.05, compared with the control group. Each assay was performed in five replicates