Table 1 Primer sequences for SCN9A proximal promoter region
Target gene | No. | Primers | Primer sequence |
|---|---|---|---|
SCN9A | 1 | Forward | 5′-ATGAAATGGTGCTGCCTACA-3’ |
Reverse | 5′-CCTGGGTGGAAGTGAAGAAA-3’ | ||
2 | Forward | 5′-ATGGTGCTGCCTACATTCAAG-3’ | |
Reverse | 5′-TGGGTGGAAGTGAAGAAAGG-3’ | ||
3 | Forward | 5′-CAACACACACACACACATGGA-3’ | |
Reverse | 5′-TGCACAAGGGCTTTTACTCTG-3’ | ||
4 | Forward | 5′-GAGTCCCAGGCCATGAAAT-3’ | |
Reverse | 5′-TATCCAGTCTTTGGGGATGC-3’ | ||
5 | Forward | 5′-GGGTGTTGTAGGTCACATTGG-3’ | |
Reverse | 5′-ATGAGGGCAAGGGACTGTTT-3’ | ||
6 | Forward | 5′-TGAAATGGTGCTGCCTACAT-3’ | |
Reverse | 5′-TGGGTGGAAGTGAAGAAAGG-3’ | ||
7 | Forward | 5′-AATGGTGCTGCCTACATTCA-3’ | |
Reverse | 5′-CCTGGGTGGAAGTGAAGAAA-3’ | ||
8 | Forward | 5′-GGGTGTTGTAGGTCACATTGG-3’ | |
Reverse | 5′-AGGGCAAGGGACTGTTTTGT-3’ | ||
9 | Forward | 5′-TGGTGCTGCCTACATTCAAG-3’ | |
Reverse | 5′-TGGGTGGAAGTGAAGAAAGG-3’ |