Fig. 4

Localization of the expression of GCα isoforms in murine brain. In situ hybridization was performed on sections of adult mouse brain using GC-1α- (a, d, g, j, m and p) and GC-2α- (b, e, h, k, n and q) specific antisense probes, as well as sense probes (c, f, i, l and o) as a negative control (results obtained with the GC-2α sense probe were similar to those obtained with the GC-1α sense probe and are not shown here). Representative sections of the cerebrum (coronal sections of the cerebral cortex: panels a-c, sagittal view of part of the limbic system: panels d-f, saggital view of hippocampus: panels g-i) and the cerebellum (j-q) are shown. GC-1α and GC-2α were expressed in cerebral cortex neurons (layer II/III and the pyramidal layer of the piriform cortex), third ventricle of the brain (epithelial cells of the choroid plexus), thalamus (medial habenulae), hippocampus (granular cell layer of the dentate gyrus and in the pyramidal layer of the cornu ammonis), and cerebellum (Purkinje cell layer and Golgi cells of the granular layer, with lower levels detectable in granule cells). GL: granular layer, ML: molecular layer, PCL: Purkinje cell layer, GN: Golgi neuron, PC: Purkinje cell, CA1-3: pyramidal layer of regions 1-3 of the hippocampal cornu ammonis, DG: hippocampal dentate gyrus, CP: choroid plexus, HA: medial habenula, HI: hippocampus. Scale bars: black: 200 μm, blue: 80 μm, white: 10 μm