Fig. 1

Induction (loss of righting reflex, LORR) and emergence (return of righting reflex, RORR) protocol for mice receiving sevoflurane. During the induction phase, the chamber sevoflurane concentration was increased to 2% for 30 s then rapidly decreased to 0.6%. Next, the sevoflurane dose was increased stepwise (0.1% every 20 min) up to 1.6%, then decreased to 1.0% by stepwise decreases (0.1% every 20 min) until all mice regained the righting reflex. The total duration of the experiment was 360 min, with an induction phase of 220 min and an emergence phase of 140 min