Fig. 1

Schematic protocol of the animal groups were the line is a non linear timeframe of seven days. The open boxes indicate a period of anesthesia alone, gray boxes a period or RIPC and black boxes a period of renal ischemia. Animals were randomly allocated into six groups: sham, control (IRI), RepISO (daily isoflurane anesthesia), RIPC (single dose isoflurane anesthesia and single dose RIPC), RepISO + RIPC (7-day isoflurane anesthesia and single dose RIPC) and RepISO + RepRIPC (7-day isoflurane anesthesia with 7-day RIPC). RIPC was applied by 3×5 min of cuff inflation on both thighs