Figure 2

Bicuculine increases neuronal viability and reduces apoptosis in sevoflurane neurotoxicity. NSCs were incubated with bicuculine before sevoflurane treatment. The cells were then labeled by Annexin V-FITC and propidium iodide (PI) in Group S and Group S + B. The apoptosis was determined by flow cytometry. A: Q1 and Q3 quadrants were represented for normal cells and death cells with red color; Q2 and Q4 quadrants were represented for viable apoptotic cells and non-viable apoptotic cells with blue color. B: The histogram represents the percentage of apoptotic cells. Compared with 0 h in Group S, *P <0.05. In Group S + B, compared with the corresponding time point in Group S, #P <0.05, ##P <0.05 and &P <0.05. Data are representative of 3 independent experiments.