Figure 2

Research methodology sequence diagram. The inclusion criteria are as follows: Age ≥ 18 yrs and remain ventilated for ≥ 48 hours; present with documented sepsis or high clinical suspicion of sepsis. Patients with head injuries, burns, spinal injuries, and multiple fractured lower limbs, those with septic shock unresponsive to treatment, moribund or with expected mortality within 48 hours will be excluded.